Biochemical Techniques

Chromatography is generally used for the separation of mixtures based on the differences in their

Ion-exchange chromatography is used for the separation of

Which chromatographic technique depends on the highly specific interactions between pairs of biological materials such as enzyme-substrate?

The general expression for the appearance of a solute in an effluent is (where V is the elution volume of a substance , V0 void volume, kD distribution constant and Vi internal water volume)

In gel filtration chromatography, separation of proteins are based on their

A size and net charge

B size and shape

C size and specific affinity

D shape and net charge

Protein separation techniques are often based on the following properties except

Which polymer is often used for matrix materials in Affinity chromatography?

In anion exchange chromatography,

In SDS -PAGE , proteins are separated on the basis of their