Genetic Technology part 2

Presentation

•

Biology

•

12th Grade

•

Medium

•

NGSS

HS-LS1-1, HS-LS3-2

Standards-aligned

Leonita Swandjaja

Used 1+ times

FREE Resource

51 Slides • 30 Questions

Multiple Choice

The process of making changes in the DNA code of a living organism is called

Selective Breeding

Inbreeding

Genetic Engineering

Di-hybrid Cross

Multiple Choice

What is an advantage of using transgenic bacteria to produce human proteins?

The bacterial proteins work better

The proteins can also be used to make plastics

The bacteria can produce human proteins in large amounts

The proteins are more durable when made by bacteria

Multiple Choice

Restriction Enzymes come from . . .

viruses

bacteria

goats

spiders

Multiple Choice

When using restriction enzymes, what is used to glue together DNA fragments

ligases

plasmids

bacteriophages

Multiple Choice

In order to insert a human gene into plasmid, both must

code for the same gene product.

be cut by the same restriction enzyme.

originate from the same type of cell.

have identical sequences.

Multiple Choice

Restriction Enzymes . . .

cut DNA at precise locations

help bacteria to pick up DNA

move DNA through a gel

copy DNA

Multiple Choice

How many restriction sites appear in the picture?

Multiple Select

Which RE's from above make sticky cuts?

Alul

HaeIII

BamHI

HindIII

EcorRI

Multiple Select

Which RE's from above make blunt cuts?

Alul

HaeIII

BamHI

HindIII

EcorRI

Multiple Choice

Which of the following is/are true about reverse transcriptase

works backwards, from DNA to RNA

works forward from RNA to DNA

works backwards from RNA to DNA

works forward from DNA to RNA

Multiple Choice

the function of reverse transcriptase is enzyme used to generate complementary DNA from an RNA template

false

true

Multiple Choice

mRNA is converted to cDNA by ………………

Ligase enzyme

Reverse Transcriptase enzyme

Restriction Endonuclease enzyme

RNA polymerase enzyme

Multiple Choice

What is a cDNA?

collection of total genomic DNA

non-coding RNA molecule (containing about 22 nucleotides) found in plants,

complementary DNA molecules synthesized from mRNA

small interfering Ribonucleic Acid, is a class of double-stranded RNA molecules

Multiple Choice

What is recombinant DNA

DNA from 2 different organisms combined

DNA from 2 different ribosomes

DNA that is separated out from a vector

Multiple Choice

Which of the following could be used as a vector for a human gene?

Bacterial Plasmid

Bacteria

A mitochondria

mRNA

Multiple Choice

A circular piece of DNA, found in bacteria, is called:

A plasmid

mRNA

Ribosome

Nucleotide

Multiple Choice

When DNA is manipulated and moved from one source to another it is known as

genetic engineering

electrophoresis

gene therapy

GMO

Multiple Choice

Plasmids, yeast, and viruses are known as ____________.

restriction enzymes

vectors

genetically modified organisms

bacteria

Multiple Choice

The diagram below shows stages in the production of a substance, such as insulin, by genetic engineering. Which line in the table below correctly identifies S and T?

S - Gene, T - Plasmid

S - Gene, T - Bacterium

S - Chromosome, T - Plasmid

S - Chromosome, T - Bacterium

Multiple Choice

________ are used to ________ DNA pieces by ________

Restriction enzymes, connect, fission

Restriction enzymes, isolate, "snipping"

Plasmids, connect, fission

Plasmids, isolate, "snipping"

Multiple Choice

Why did scientists edit the genes of bacteria to produce insulin?

Because the bacteria were diabetic

Because bacteria have simple DNA that is easier to manipulate, making it easy to produce insulin in a lab

Because bacteria's complex DNA was perfect for insulin production

Because insulin has no use outside of a lab setting

Multiple Choice

What does the repressor protein do?

Allows RNA polymerase to read the gene

Prevents RNA polymerase from reading the gene

Reads the gene

Translates the gene

Multiple Choice

What is an operon?

a part of the promoter region of a gene

a part of RNA polymerase

a unit made up of linked genes that code for proteins needed to do a specific task

A protein needed for DNA replication

Multiple Choice

In the lac operon, the genes within the operon will be expressed if:

lactose is absent in the cell

galactose is present in the cell

lactose is present in the cell

glucose is absent in the cell

Multiple Choice

What type of operon is the lac (lactose) operon?

inducible

repressible

positive control

promoible

Multiple Choice

Which of the following is an example of post-transcriptional control of gene expression?

the addition of methyl groups to cytosine bases of DNA

the binding of transcription factors to a promoter

the removal of introns and splicing together of exons

gene amplification contributing to cancer

Multiple Choice

In order for a gene to be expressed, ___ must bind to the gene's ___, a specific sequence of nucleotides at one end of the gene, thus initiating transcription.

DNA polymerase, promotor

DNA polymerase, operator

RNA polymerase, promotor

RNA polymerase, operator

Multiple Choice

What is the inducer in the lac operon?

cAMP

glucose

lactose

CRP

Multiple Choice

In eukaryotes, proteins that help the RNA polymerase bind to the regulatory region for transcription are called ____________.

transcription factors

topoisomerase

regulatory genes

activators

Multiple Choice

Gene regulation is the process that is used to:

help make sugars for cells in plants.

make glycosomes in cells.

turn genes on and off.

Not sure, need help.

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