Gene Cloning

Which is a use of genetically engineered bacteria?

Several procedures involved in the creation of a transgenic organism are listed.

1. A specific gene of interest is identified in DNA.

2. The DNA fragment is placed in a plasmid.

3. The DNA fragment of interest is isolated.

4. The plasmid is transferred to a bacterial cell.

5. A plasmid is cut by a restriction enzyme.

Which selection represents the correct order of events in creating a transgenic organism?