K10MS W10 LAC OPERON TUTORIAL
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•
Biology
•
12th Grade
•
Medium
NUR BM
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13 Slides • 14 Questions
1
K10MS W10 LAC OPERON TUTORIAL
by NUR BM
LO: explain the concept of operon and gene regulation
2
Multiple Choice
which of the following is NOT the component of lac operon?
structural genes
repressor protein
promoter
operator
3
Fill in the Blanks
Type answer...
4
name and describe structure of each component of lac operon present based on the provided diagram
lac operon
5
6
7
Fill in the Blanks
Type answer...
8
Multiple Choice
which of the following enzyme produced when transcription of lac Y is successful?
transacetylase
RNA polymerase
B galactosidase
lactose permease
9
structural genes
explain how the structural genes are controlled as single unit.
10
structural gene contains only 1 promoter
RNA polymerase binds once and transcribed all structural genes at once producing single mRNA strand
advantage? increase efficiency of enzyme synthesise
11
Fill in the Blanks
Type answer...
12
Multiple Choice
which of the following process did not occur during lac operon expression?
transcription
mRNA synthesis
protein synthesis
DNA replication
13
Multiple Choice
single unit of gene expression increase efficiency of lactose metabolism in E. coli as follows EXCEPT ?
mRNA is synthesized consecutively during each transcription
the single transcribed mRNA has the code to synthesize all enzymes at once
each structural genes contains separate promoter
RNA polymerase binds at single promoter to transcribe all genes at once
14
RNA polymerase
explain its function and how it is required in lactose operon expression
15
RNA polymerase
RNA polymerase catalyze the transcription of structural genes (lac Z, lac Y and lac A)
binds itself on promoter site when required
16
Multiple Choice
which of the following is the binding site of RNA polymerase
operator
repressor protein
promoter
structural genes
17
Fill in the Blanks
Type answer...
18
respressor protein
explain how the function of repressor protein is important in controlling the lac operon expression
19
Repressor protein
repressor protein binds on the operator
it blocks RNA polymerase from transcribing the structural genes
20
Fill in the Blanks
Type answer...
21
Multiple Choice
Which statement about Escherichia coli which has regulatory gene that has undergone mutation is true?
Only β-galactosidase is produced
Repressor produced binds with operator gene.
RNA polymerase is unable to bind with promoter gene
E. coli produces β-galactosidase, permease and transacetylase continuously.
22
ALLOLACTOSE
describe how allolactose is produced and its primary role
23
ALLOLACTOSE
allolactose is isomer of lactose
produced only when lactose is present (individual drinks milk)
binds to repressor protein
inactivate repressor protein by altering its confirmation
repressor protein can no longer binds to operator
24
Multiple Choice
In the lactose operon, both genes P and Q undergo the transcription process. Gene Q can only undergo transcription with the existence of lactose and at the same time, gene P product is released from R. Which of the following is true about the genes P, Q and R?
P Regulator gene Q Structural gene
R Operator gene
R Regulator gene P Structural gene
Q Operator gene
R Regulator gene Q Structural gene
P Operator gene
P promoter gene Q Regulator gene
R Operator gene
25
Multiple Choice
Which of the following statements are true about lactose operon in the presence of lactose?
I. The regulator gene produces an active repressor molecule.
II. Lactose binds with the repressor molecule.
III. The inactive repressor molecule binds to the promoter.
IV. The RNA polymerase binds to the operator.
V. Transcription of structural gene occurs.
I, II and III
I, II and V
I, III and IV
I, II, IV and V
26
27
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K10MS W10 LAC OPERON TUTORIAL
by NUR BM
LO: explain the concept of operon and gene regulation
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