Cloning Lesson Lesson

11 Slides • 10 Questions

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Multiple Choice

Restriction Enzymes can cut any sequence of DNA.

1

True

2

False

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Dropdown

DNA is created by using the

from a bacteria cell and splicing a section of DNA into it.

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Fill in the Blanks

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Multiple Choice

The unpaired nucleotides produced by the action of restriction enzymes are referred to as.....

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sticky ends

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single strands

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restriction fragments

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Multiple Select

Restriction endonucleases....

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Cut DNA at a specific sites

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Can be used to isolate a gene of interest

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Protect bacterial cells by cutting foreign DNA.

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Reorder

The following steps involved in gene cloning procedures. Rearrange them in descending order.

Recombinant DNA is inserted into a cell for replication.

Insert DNA fragment into the cut bacterial plasmid.

Cleave the plasmid and human DNA with the same restriction enzyme.

Isolate the bacterial plasmid and desired gene.

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Multiple Select

Sticky ends are produced when the restriction enzyme cuts...

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in a staggered manner

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straight through the DNA

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Match

Match the following

Plasmid

Genetic engineering

Restriction enzyme

Recombinant DNA

small circular DNA molecule

the direct manipulation of genes for practical purpose

enzyme that cuts DNA at specific recognition sites

DNA formed by combining DNA from different sources

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Multiple Select

DNA Cloning is used in which of the following technologies?

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Gel Electrophoresis

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Polymerase Chain Restriction

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DNA Sequencing

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DNA Mapping

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Fill in the Blanks

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