

DNA Cloning in Agriculture
Presentation
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Science
•
University
•
Easy
Annie Jeyachristy Sam
Used 4+ times
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22 Slides • 28 Questions
1
By Annie Jeyachristy Sam
DNA Cloning in Agriculture: Shaping the Future of Sustainable Crop Improvement
Dr. Sam Annie Jeyachristy
Royal College of Medicine Perak, Universiti Kuala Lumpur (UniKL RCMP)
Ipoh, Perak, Malaysia
2
Poll
Mood check:
How are you feeling today?
🤩 Feeling great
😊 Doing okay
😓 Struggling a bit
🫠 Feeling overwhelmed
3
Describe the basic steps involved in DNA cloning.
Recognize the use of DNA cloning in agricultural crop improvement.
Identify the benefits and challenges of DNA cloning in sustainable farming.
Recall real-world examples of genetically modified crops produced through cloning.
4
Multiple Choice
What does genetic engineering generally involve?
Crossing two plants to get better yield
Using fertilizers to improve plant growth
Altering the DNA of an organism to produce desired traits
Studying the behavior of insects in fields
5
Multiple Choice
What is the primary function of DNA in living organisms?
Provide energy for cellular functions
Store genetic information
Help in photosynthesis
Maintain body temperature
6
Multiple Choice
Which of the following best describes a gene?
A type of fertilizer
A segment of DNA that codes for a protein
A cell organelle that produces energy
A plant hormone
7
Multiple Choice
What is a plasmid?
A root structure in legumes
A small circular DNA molecule used in genetic engineering
A type of soil bacteria
A type of gene found only in plants
8
Multiple Choice
Which of the following is a genetically modified (GM) crop?
Bt Cotton
Wild rice
Organic wheat
Natural maize
9
Importance in agriculture
Crop improvement, pest resistance, etc.
Definition
Production of identical copies of a DNA fragment.
Purpose
Amplify genes or DNA segments for research or application.
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11
Multiple Choice
DNA cloning is the process of
Destroying DNA fragments
Combining two plants together
Extracting RNA from a cell
Producing identical copies of a DNA fragment
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14
Multiple Choice
Which method is commonly used for DNA purification?
PCR
UV radiation
Spin columns
Alcohol evaporation
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18
Multiple Choice
Which of the following is commonly used as a cloning vector?
mRNA
Ribosome
Enzyme
Plasmid
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20
Multiple Choice
Which of the following is NOT a tool used in DNA cloning?
Restriction enzymes
DNA ligase
Vectors
Ribosomes
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23
Multiple Choice
Restriction enzymes cut DNA at
Random positions
Specific recognition sites
All adenine bases
Telomeres
24
Multiple Choice
What enzyme is responsible for joining DNA fragments?
Restriction enzyme
DNA ligase
RNA polymerase
Helicase
25
Multiple Choice
Which of the following is a common host used in cloning?
Streptococcus
E. coli
Mycobacterium
Rhizobium
26
Multiple Choice
Antibiotic resistance genes are used to
Kill all bacteria
Destroy foreign DNA
Identify successful transformants
Promote mutation
27
Multiple Choice
Which of the following is a restriction enzyme?
Taq polymerase
EcoRI
pUC19
GFP
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Summary of DNA Cloning
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30
Multiple Choice
What technique is used to confirm the presence of the insert?
Western blot
Southern blot
Colony PCR
SDS-PAGE
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33
Watch this documentary film (52 min) in your free time.
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38
Multiple Choice
Bt Brinjal is an example of
Chemical hybrid
GM crop with insect resistance
Organic farming
Fungicide-treated plant
39
Multiple Choice
What is used to isolate plasmid DNA from clones?
Phenol wash
Miniprep kit
Ethanol gel
Taq polymerase
40
Multiple Choice
Which tool helps map genes on a plasmid?
Microsoft Excel
AutoCAD
Plasmid mapping software
Zoom
41
Multiple Choice
The Cartagena Protocol deals with
Soil fertility
Organic certification
Biosafety of GMOs
Export taxes
42
Multiple Choice
CRISPR is associated with
Bacterial respiration
Gene editing
Viral infection
Protein folding
43
Multiple Choice
Bt cotton contains a gene from
Bacillus thuringiensis
E. coli
Rhizobium
Yeast
44
Multiple Choice
DNA cloning has no role in agriculture.
True
False
45
Multiple Choice
Blue colonies in blue-white screening contain the desired insert.
True
False
46
Multiple Choice
Which is a benefit of DNA cloning in agriculture?
Increasing pesticide use
Reducing plant growth
Promoting genetic instability
Improving crop nutritional content
47
Multiple Choice
One major public concern about GM crops is
Higher cost of fertilizer
Food safety and ethics
More water usage
Loss of color in crops
48
Multiple Choice
Which of the following summarizes the goal of DNA cloning?
Remove all genes
Replicate and use specific genes effectively
Cause mutations
Delay plant growth
49
Poll
Mood check:
How are you feeling today?
🤩 Feeling great
😊 Doing okay
😓 Struggling a bit
🫠 Feeling overwhelmed
50
By Annie Jeyachristy Sam
DNA Cloning in Agriculture: Shaping the Future of Sustainable Crop Improvement
Dr. Sam Annie Jeyachristy
Royal College of Medicine Perak, Universiti Kuala Lumpur (UniKL RCMP)
Ipoh, Perak, Malaysia
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