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DNA Cloning in Agriculture

DNA Cloning in Agriculture

Assessment

Presentation

•

Science

•

University

•

Easy

Created by

Annie Jeyachristy Sam

Used 4+ times

FREE Resource

22 Slides • 28 Questions

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By Annie Jeyachristy Sam

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DNA Cloning in Agriculture: Shaping the Future of Sustainable Crop Improvement

Dr. Sam Annie Jeyachristy

Royal College of Medicine Perak, Universiti Kuala Lumpur (UniKL RCMP)
Ipoh, Perak, Malaysia

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Poll

Mood check:

How are you feeling today?

🤩 Feeling great

😊 Doing okay

😓 Struggling a bit

🫠 Feeling overwhelmed

3

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  • ​Describe the basic steps involved in DNA cloning.

  • Recognize the use of DNA cloning in agricultural crop improvement.

  • Identify the benefits and challenges of DNA cloning in sustainable farming.

  • Recall real-world examples of genetically modified crops produced through cloning.

​Learning Objectives

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Multiple Choice

What does genetic engineering generally involve?

1

Crossing two plants to get better yield

2

Using fertilizers to improve plant growth

3

Altering the DNA of an organism to produce desired traits

4

Studying the behavior of insects in fields

5

Multiple Choice

What is the primary function of DNA in living organisms?

1

Provide energy for cellular functions

2

Store genetic information

3

Help in photosynthesis

4

Maintain body temperature

6

Multiple Choice

Which of the following best describes a gene?

1

A type of fertilizer

2

A segment of DNA that codes for a protein

3

A cell organelle that produces energy

4

A plant hormone

7

Multiple Choice

What is a plasmid?

1

A root structure in legumes

2

A small circular DNA molecule used in genetic engineering

3

A type of soil bacteria

4

A type of gene found only in plants

8

Multiple Choice

Which of the following is a genetically modified (GM) crop?

1

Bt Cotton

2

Wild rice

3

Organic wheat

4

Natural maize

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Importance in agriculture

Crop improvement, pest resistance, etc.

Definition

Production of identical copies of a DNA fragment.

Purpose

Amplify genes or DNA segments for research or application.

​Introduction

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Multiple Choice

DNA cloning is the process of

1

Destroying DNA fragments

2

Combining two plants together

3

Extracting RNA from a cell

4

Producing identical copies of a DNA fragment

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Multiple Choice

Which method is commonly used for DNA purification?

1

PCR

2

UV radiation

3

Spin columns

4

Alcohol evaporation

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18

Multiple Choice

Which of the following is commonly used as a cloning vector?

1

mRNA

2

Ribosome

3

Enzyme

4

Plasmid

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20

Multiple Choice

Which of the following is NOT a tool used in DNA cloning?

1

Restriction enzymes

2

DNA ligase

3

Vectors

4

Ribosomes

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Multiple Choice

Restriction enzymes cut DNA at

1

Random positions

2

Specific recognition sites

3

All adenine bases

4

Telomeres

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Multiple Choice

What enzyme is responsible for joining DNA fragments?

1

Restriction enzyme

2

DNA ligase

3

RNA polymerase

4

Helicase

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Multiple Choice

Which of the following is a common host used in cloning?

1

Streptococcus

2

E. coli

3

Mycobacterium

4

Rhizobium

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Multiple Choice

Antibiotic resistance genes are used to

1

Kill all bacteria

2

Destroy foreign DNA

3

Identify successful transformants

4

Promote mutation

27

Multiple Choice

Which of the following is a restriction enzyme?

1

Taq polymerase

2

EcoRI

3

pUC19

4

GFP

28

​Summary of DNA Cloning

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Multiple Choice

What technique is used to confirm the presence of the insert?

1

Western blot

2

Southern blot

3

Colony PCR

4

SDS-PAGE

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Watch this documentary film (52 min) in your free time.​

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Multiple Choice

Bt Brinjal is an example of

1

Chemical hybrid

2

GM crop with insect resistance

3

Organic farming

4

Fungicide-treated plant

39

Multiple Choice

What is used to isolate plasmid DNA from clones?

1

Phenol wash

2

Miniprep kit

3

Ethanol gel

4

Taq polymerase

40

Multiple Choice

Which tool helps map genes on a plasmid?

1

Microsoft Excel

2

AutoCAD

3

Plasmid mapping software

4

Zoom

41

Multiple Choice

The Cartagena Protocol deals with

1

Soil fertility

2

Organic certification

3

Biosafety of GMOs

4

Export taxes

42

Multiple Choice

CRISPR is associated with

1

Bacterial respiration

2

Gene editing

3

Viral infection

4

Protein folding

43

Multiple Choice

Bt cotton contains a gene from

1

Bacillus thuringiensis

2

E. coli

3

Rhizobium

4

Yeast

44

Multiple Choice

DNA cloning has no role in agriculture.

1

True

2

False

45

Multiple Choice

Blue colonies in blue-white screening contain the desired insert.

1

True

2

False

46

Multiple Choice

Which is a benefit of DNA cloning in agriculture?

1

Increasing pesticide use

2

Reducing plant growth

3

Promoting genetic instability

4

Improving crop nutritional content

47

Multiple Choice

One major public concern about GM crops is

1

Higher cost of fertilizer

2

Food safety and ethics

3

More water usage

4

Loss of color in crops

48

Multiple Choice

Which of the following summarizes the goal of DNA cloning?

1

Remove all genes

2

Replicate and use specific genes effectively

3

Cause mutations

4

Delay plant growth

49

Poll

Mood check:

How are you feeling today?

🤩 Feeling great

😊 Doing okay

😓 Struggling a bit

🫠 Feeling overwhelmed

50

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pattern-tertiary

By Annie Jeyachristy Sam

media

DNA Cloning in Agriculture: Shaping the Future of Sustainable Crop Improvement

Dr. Sam Annie Jeyachristy

Royal College of Medicine Perak, Universiti Kuala Lumpur (UniKL RCMP)
Ipoh, Perak, Malaysia

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