Gene Cloning
Presentation
•
Biology
•
12th Grade
•
Hard
Joseph Anderson
FREE Resource
11 Slides • 10 Questions
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Multiple Choice
Restriction Enzymes can cut any sequence of DNA.
True
False
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Dropdown
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Fill in the Blanks
What enzyme is used to ensure that the recombinant DNA created is bonded together along the sugar-phosphate backbone?
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Multiple Choice
The unpaired nucleotides produced by the action of restriction enzymes are referred to as.....
sticky ends
single strands
restriction fragments
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Multiple Select
Restriction endonucleases....
Cut DNA at a specific sites
Can be used to isolate a gene of interest
Protect bacterial cells by cutting foreign DNA.
15
Reorder
The following steps involved in gene cloning procedures. Rearrange them in descending order.
Recombinant DNA is inserted into a cell for replication.
Insert DNA fragment into the cut bacterial plasmid.
Cleave the plasmid and human DNA with the same restriction enzyme.
Isolate the bacterial plasmid and desired gene.
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Multiple Select
Sticky ends are produced when the restriction enzyme cuts...
in a staggered manner
straight through the DNA
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Match
Match the following
Plasmid
Genetic engineering
Restriction enzyme
Recombinant DNA
small circular DNA molecule
the direct manipulation of genes for practical purpose
enzyme that cuts DNA at specific recognition sites
DNA formed by combining DNA from different sources
small circular DNA molecule
the direct manipulation of genes for practical purpose
enzyme that cuts DNA at specific recognition sites
DNA formed by combining DNA from different sources
18
Multiple Select
DNA Cloning is used in which of the following technologies?
Gel Electrophoresis
Polymerase Chain Restriction
DNA Sequencing
DNA Mapping
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Fill in the Blanks
In DNA cloning, the plasmid acts as the
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