BIOKEEN CHAPTER 7 (Set A)
Presentation
•
Biology
•
12th Grade
•
Practice Problem
•
Easy
PDT BIOLOGI
Used 2+ times
FREE Resource
1 Slide • 12 Questions
1
Multiple Choice
1. What is DNA recombinant technology?
Techniques for sequencing and manipulating gene
Techniques in carrying fragment DNA into plasmid
Joining together DNA molecules of 2 different sources to produce new genetic combinations
Joining a plasmid to the host cell to produce new organism
2
Multiple Choice
2. These are the tools used in recombinant DNA technology EXCEPT
Modifying enzymes
Target RNA
Host cell
Restriction enzymes
3
RECOMBINANT DNA TECHNOLOGY
Answer questions no. 3 - 9 based on this diagram.
Learning outcomes7.1 Recombinant DNA Technology
b)
Lists tools used in recombinant DNA technology and state their function
10
4
Multiple Choice
3. What is target DNA?
Gene to be copied
Gene to be cloned
Gene to be destroyed
Gene to be inserted into host cell
5
Fill in the Blanks
4. Bacteria that are resistance to bacteriophage infection naturally has
6
Fill in the Blanks
5. BamH1 is an example of restriction enzyme which produced by bacteria named
7
Fill in the Blanks
6. DNA cloning vector is an agent DNA molecule that carries _______ ___ ________ into a host cell.
8
Multiple Choice
7. Examples of DNA cloning vectors as below EXCEPT
Bacteriophage
Cosmid
YAC
Bacteria
9
Multiple Choice
8. Host cell must be
able to replicate independently in the vector
able to accept foreign DNA in MCS
able to accept recombinant DNA by transformation
Has selectable marker gene
10
Fill in the Blanks
9. This enzyme can joins separate 2 DNA fragments and catalyzes the formation of phosphodiester bond.
11
Multiple Choice
10. What is the purpose of using PCR?
To amplify a small amount of DNA
To duplicate the DNA
To cut/cleave the DNA
To denature the double stranded of DNA
12
Multiple Choice
11. Choose the correct step in PCR.
Denaturation : temperature 500C
Elongation : temperature 720C
Annealing : temperature 900C
13
Fill in the Blanks
12. Name the enzyme that catalyzes the synthesis of new DNA strand by adding free nucleotides in PCR.
1. What is DNA recombinant technology?
Techniques for sequencing and manipulating gene
Techniques in carrying fragment DNA into plasmid
Joining together DNA molecules of 2 different sources to produce new genetic combinations
Joining a plasmid to the host cell to produce new organism
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