Post-Test MT Day 3

1. TNC Recovery - Blood Products

• ​Pre-Processing TNC

  • 871.4 x 10^6

• Post-Processing TNC

  • 574 x 10^6

• Final Product Volume

  • 23 ml

• WBC Count Post

  • 28.7 x 10^6

2. Cell Recovery

• Cell Number Before Cryopreservation

  • 25 x 10^6

• Cell Number After Thawing

  • 18.9 x 10^6

Calculate cell recovery!

3. Cell Viability

• Total cells

  • 187.9 x 10^6

• Viable cells

  • 175.8 x 10^6

Calculate cell viability!

4. Cell Viability

• Viable cells

  • 205.2 x 10^6

• Viabilty

  • 98%

Calculate total cells!

5. Cell Dilution #1

• Total cells

  • 17.9 x 10^6/ml

• Viable cells

  • 14.3 x 10^6/ml

• Viability

  • 86%

• Suspension volume

  • 2 ml

• Calculate:

  • Product: 20 x 10^6 in 4 ml NaCl

  • QC: 3 x 10^6 in 3 ml NaCl

6. Cell Dilution #2

• ​Total cells

  • 63.4 x 10^6/ml

• Viable cells

  • 62.6 x 10^6/ml

• Viability

  • 99%

• Suspension volume

  • 7.7 ml

• Calculate:

  • Product: 60 x 10^6 in 50 ml NaCl

  • QC: 3 x 10^6 in 3 ml NaCl

  • Cryo: 400 x 10^6 in 400 ml Cryo Medium. Dosage:

    • 150 x 10^6 (large cryobag)

    • 25 x 10^6 (small cryobag)