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Dehydration

Dehydration

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Dehydration

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26.

Considerable shrinkage, Hard-
ens tissue more than ethanol
does

Aside from being limited to small pieces
of tissue, volatility, more volume is re-
quired, what are the (2) reasons why
Acetone is not recommended for routine
use?

27.

1) 2 to 3 changes
2) about 2x a week

Acetone Precautions:

1) ___ changes should be used
2) should be changed frequently, about
___

28.

Diethylene Dioxide (Dioxane)

It is an excellent dehydrating agent,
which can also be used as clearing agent
known for its generally good miscibility
with water and paraffin, as well as its
capability of being placed directly into
the solution after washing

29.

Dioxane is Expensive

Aside from not being able to easily pro-
duce a good ribbon, as well as having
toxic and dangerous vapor, what is the
other disadvantage that Dioxane has?

30.

Well-ventilated, Washed down
the sink TOTALLY

Because Acetone is toxic, the room
should be ___, and residues should be
___

31.

Graupner's Method (7 Hours)

A combination of pure dioxane solution
and paraffin wax embedded in mold and
cool in water, also, how much time is
allotted in this method?

32.

Weiseberger's Method (3-24
Hours)

The tissue is wrapped in a gauze bag and
suspended in a bottle containing diox-
ane and a little anhydrous calcium oxide,
also how much time is allotted in this
method?

33.

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