​Imagine Prince Priyanshu  is a super scientist and he puts TWO questions to FY BT kingdom

​How did Tswett separate the  5 bands in his experiment ?

​Why did Tswett get 5 bands in his experiment ?

​Cue Card

Initially all the athletes started the race from the start position. As the race starts, slowly there is separation  between them and one who runs the fastest  reaches the goal first and one who is the slowest  reaches the goal last. And there are others in  between the two extremes because of their  different speeds.​

​Answer to Prince Priyanshu's first question

​the five solutes separated on the column because of the difference in their migration rates or velocities

mig​ration rate increases from chlorophyll b to carotene.

​But why does carotene travel the fastest and chlorophyll b was the slowest when all the five solute travel across the column under similar experimental condition ???

Process of adsorption

​It is a surface phenomenon in which the solutes (adsorbates) adsorb on the surface of another solute (adsorbent)

​The process depends on

​1. the properties of surface of an adsorbent

​2. the properties of adsrobates

​Can we answer Prince Priyanshu 2nd Question

​the five solutes have different migration rates across the column of CaCo₃ because they have different degrees of adsorption on the surface of CaCo₃

​Can we say carotene has highest migration rate but least degree of adsorption on CaCO₃ whereas chlorophyll b has the slowest migration rate but highest degree of adsorption on CaCO3

​Adsorption Column Chromatography