Enzyme Immobilization

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Biology

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Practice Problem

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Medium

Lim Wei

Used 4+ times

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11 Slides • 13 Questions

Enzyme Immobilization

Restriction of enzyme mobility in a fixed space

Advantages of enzyme immobilization

Enzyme reutilization
Elimination of enzyme recovery and purification processes
Provide a better environment for enzyme activity
Reduce effluent handling problems
Improve purity of enzyme

Matrix Entrapment

Enzyme Immobilization: Entrapment

Open Ended

Define in your own words what is matrix entrapment

Type response here

Multiple Choice

Are matrix entrapments normally used for polymeric materials?

True

False

Multiple Select

Select ALL the materials that are commonly used as matrices for entrapment

Cellulose Triacetate

Water

Agar

Alginate

Multiple Choice

Which of the following is NOT an advantage for matrix entrapment?

Low Cost

Easy handling

Can be practised at a large scale

Less chance of conformational changes in enzyme

Membrane Entrapment

Enzyme Immobilization: Entrapment

Open Ended

Define in your own words what is membrane entrapment

Type response here

Multiple Choice

Which of the following shows membrane entrapment?

Multiple Choice

Which of the following is the advantage of membrane entrapment?

No diffusion barrier

Low leakage

Continuous product formation

Multiple Choice

Which of the following is the disadvantage of membrane entrapment?

Catalytic activity is unaffected by chemical reaction

Diffusional limitation

Larger surface area for reaction

Poll

Can chemical reaction interfere the enzyme activity?

Yes

Adsorption & Covalent Binding

Enzyme Immobilization: Bound

Fill in the Blanks

Type answer...

Multiple Choice

Which of the following diagram represents a covalent-bounded enzyme immobilization?

Multiple Choice

Which of the following is considered as a disadvantage to the adsorption method of immobilization?

It is possible to separate and purify the enzymes while being immobilized

The enzymes are not usually deactivated by adsorption

The adsorption is a reversible process

State of immobilization is very sensitive to solution pH, ionic strength and

temperature

Multiple Choice

Below are the advantages of adsorption in immobilised enzyme systems, except:

No desorption problem

Easy to carry out

No reagents required

Cheap method

Experimental Method for Immobilizing Enzymes

Enzyme Immobilization

Restriction of enzyme mobility in a fixed space

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