MPA 8 Effect of pH on enzyme action
Presentation
•
Science
•
3rd - 5th Grade
•
Practice Problem
•
Medium
Michael Kavanagh
Used 18+ times
FREE Resource
5 Slides • 8 Questions
1
2
The enzyme catalase acts on the substrate hydrogen peroxide and produces water and oxygen. Yeast solution or celery juice can be used as a source of catalase.
3
Mix together chopped celery, water and pH buffer 2 in a test tube and place in a water bath at 30 C. Place hydrogen peroxide in a separate graduated cylinder in the water bath. The water bath keeps the temperature constant and pH buffer is used to maintain a fixed pH.
4
Leave both solutions in the water bath for 10 minutes to allow time for the enzyme to be affected by the pH. Add the celery extract solution to the hydrogen peroxide. Record the volume of foam produced by oxygen after 10 seconds as a measure of the speed or rate of reaction
5
Repeat the experiment at different pH‟s using different pH buffers.
The optimum pH produces the largest volume of foam over the same time. At other pH‟s the enzyme is denatured.
6
Multiple Choice
Name the substrate for the enzyme catalase.
Starch
Protein
Hydrochloric acid
Hydrogen peroxide
7
Multiple Choice
Describe a suitable source for catalase enzyme.
Celery
Starch
Protein
Hydrogen peroxide
8
Multiple Choice
State a factor that was kept constant in this investigation
Light intensity
Temperature
Distance to lamp
pH
9
Multiple Choice
How is the rate of enzyme action measured in this investigation.
Volume of foam produced.
Volume of oxygen foam produced per unit time.
Concentration of oxygen produced.
Amount of product produced
10
Multiple Choice
Name the products of the action of catalase enzyme
Water
Oxygen
Water and carbon dioxide
Water and oxygen
11
Multiple Choice
How is the pH varied in this investigation?
Different pH buffer in each graduated cylinder
The same pH buffer in each graduated cylinder
All graduated cylinders are placed in the same water bath.
Each graduated cylinder is placed in a different water bath at different temperatures.
12
Multiple Choice
How is the temperature kept constant in this investigation?
Different pH buffer in each graduated cylinder
The same pH buffer in each graduated cylinder
All graduated cylinders are placed in the same water bath.
Each graduated cylinder is placed in a different water bath at different temperatures.
13
Multiple Choice
What is meant by the optimum pH for an enzyme.
The pH it produces.
The pH it is found in
The pH that denatures the enzyme
The pH at which it works fastest
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